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A transcription factor DAF-5 functions in Haemonchus contortus development
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In: Parasit Vectors (2021)
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Abstract:
BACKGROUND: Abnormal dauer formation gene (daf-5), located downstream of the DAF-7 signalling pathway, mainly functions in dauer formation and reproductive processes in the free-living nematode Caenorhabditis elegans. Although the structure and function of daf-5 have been clarified in C. elegans, they still remain totally unknown in Haemonchus contortus, a socio-economically important parasitic nematode of gastric ruminants. METHODS: A homologue of daf-5, Hc-daf-5, and its inferred product (Hc-DAF-5) in H. contortus were identified and characterized in this study. Then the transcriptional profiles of Hc-daf-5 and the anatomical expression of Hc-DAF-5 in H. contortus were studied using an integrated molecular approach. RNA interference (RNAi) was performed to explore its function in transition from the exsheathed third-stage larvae (xL3s) to the fourth-stage larvae (L4s) in vitro. Finally, the interaction between Hc-DAF-5 and Hc-DAF-3 (a co-Smad) was detected by bimolecular fluorescence complementation (BiFc) in vitro. RESULTS: It was shown that Hc-DAF-5 was a member of the Sno/Ski superfamily. Hc-daf-5 was transcribed in all developmental stages of H. contortus, with significant upregulation in L3s. Native Hc-DAF-5 was localized in the reproductive organs, cuticle, and intestine via immunohistochemistry. RNAi revealed that specific small interfering RNAs (siRNAs) could retard xL3 development. In addition, the interaction between Hc-DAF-5 and Hc-DAF-3 indicated that the SDS box of Hc-DAF-5 was dispensable for the binding of Hc-DAF-5 to Hc-DAF-3, and the MH2 domain was the binding region between Hc-DAF-3 and Hc-DAF-5. CONCLUSIONS: In summary, these findings show that Hc-daf-5 functions in the developmental processes of H. contortus, and this study is the first attempt to characterize the daf-5 gene in parasitic nematodes. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-021-05036-2.
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Keyword:
Research
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URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507387/
https://doi.org/10.1186/s13071-021-05036-2
http://www.ncbi.nlm.nih.gov/pubmed/34641971
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Voice Onset Time in a Language Without Distinctive Voicing: Blackfoot Oral Stops
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In: Montana-Alberta Conference on Linguistics (mACOL) (2020)
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A DAF-3 co-Smad molecule functions in Haemonchus contortus development
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Articulation Speaks to Executive Function: An Investigation in 4- to 6-Year-Olds
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Bayesian data analysis in the phonetic sciences: A tutorial introduction
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Alveolar and Postalveolar Voiceless Fricative and Affricate Productions of Spanish–English Bilingual Children With Cochlear Implants
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Aligning the timelines of phonological acquisition and change
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The effect of speakers' sex on voice onset time in Mandarin stops
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Language-specific developmental differences in speech production: A cross-language acoustic study
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Language specificity in the perception of voiceless sibilant fricatives in Japanese and English: Implications for cross-language differences in speech-sound development
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Contrast and covert contrast: The phonetic development of voiceless sibilant fricatives in English and Japanese toddlers
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The Phonetic Development of Voiceless Sibilant Fricatives in English, Japanese and Mandarin Chinese
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In: http://rave.ohiolink.edu/etdc/view?acc_num=osu1228250787 (2008)
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