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MATTOS E SILVA, R. V. (1994) O português arcaico: morfologia e sintaxe. São Paulo, Ed. Contexto/CED-UFBA (Coleção Repensando a Língua Portuguesa), 138p
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In: DELTA: Documentação e Estudos em Linguística Teórica e Aplicada; v. 11, n. 1 (1995) ; 1678-460X ; 0102-4450 (2019)
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Literacy in a cultural historical perspective ; Alfabetização e letramento em uma perspectiva histórico cultural
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In: Obutchénie: Revista de Didática e Psicologia Pedagógica; 2017: v. 1, n. 3 (set./dez. 2017); 553-575 ; 2526-7647 (2017)
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“Viagem ao mundo das sílabas”: intervenção na consciência fonológica ; “Journey to the syllables' world”: intervention in phonological awareness
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Journey to the syllables’ world : intervention in phonological awareness
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A inter-relação entre o processamento auditivo e a competência leitora
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Morphological and ultrastructural analysis of sheep primordial follicles preserved in 0.9% saline solution and TCM 199
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Matos, Maria Helena Tavares de; Andrade, Evelyn Rabelo; Lucci, Carolina Madeira; Báo, Sônia Nair; Silva, José Roberto Viana; Santos, R.R.; Ferreira, Marcos Antônio Leal; Costa, Sonia Helena Furtado; Celestino, Juliana Jales de Hollanda; Figueiredo, José Ricardo de. - : Elsevier, 2004
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Abstract:
The objective was to determine the morphological and ultrastructural features of sheep primordial follicles preserved in either 0.9% saline solution or TCM 199 at different temperatures. Soon after death, the ovarian pair of each ewe (n=5) was divided into 25 fragments. One fragment was immediately fixed for morphological evaluation (control). The other 24 fragments were randomly distributed in tubes containing 2 ml of 0.9% saline solution or TCM 199 and maintained at 4, 20 or 39 °C for 2, 4, 12, or 24 h. Based on histological assessment, storage of ovarian fragments in 0.9% saline solution at 20 °C for up to 24 h and in both solutions at 39 °C for 4, 12 or 24 h increased (P<0.01) the percentage of degenerate primordial follicles compared with controls. In contrast, preservation at 4 °C in both solutions, kept the percentage of morphologically normal primordial follicles similar to control values. Although histological integrity of primordial follicles was maintained in fragments stored at 20 °C for up to 24 h in TCM 199, these results were not confirmed by ultrastructural analysis. Based on transmission electron microscopy, only primordial follicles stored at 4 °C for up to 24 h, at 20 °C for up to 12 h and at 39 °C for up to 2 h in both solutions were ultrastructurally normal. In conclusion, sheep primordial follicles were successfully preserved at 4 °C for up to 24 h, at 20 °C for up to 12 h and at 39 °C for 2 h in 0.9% saline solution or TCM 199.
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Keyword:
Morfologia (Animais); Ovino
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URL: http://repositorio.unb.br/handle/10482/17614
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Cryopreservation of caprine ovarian tissue using glycerol and ethylene glycol
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Degeneration rate of preantral follicles in the ovaries of goats
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